Effect of penicillin on the in vivo formation of the D-alanyl-L-alanine peptide cross-linkage in cell walls of Micrococcus luteus.
نویسندگان
چکیده
Penicillin G was found to inhibit the formation of the d-alanyl-l-alanine cross-linkage in intact cells of Micrococcus luteus. This reaction was approximately 50-fold less susceptible to penicillin than the formation of the d-alanyl-l-lysine cross-linkage in the same organism. The presence of two penicillin-susceptible transpeptidation reactions that function in the incorporation of peptidoglycan precursors into the cell wall is proposed.
منابع مشابه
Role of the penicillin-sensitive transpeptidation reaction in attachment of newly synthesized peptidoglycan to cell walls of Micrococcus luteus.
Cell-wall preparations of Micrococcus luteus (lysodeikticus) catalyze in vitro peptidoglycan synthesis from UDP N-acetyl-D-glucosamine, UDP N-acetylmuramic acid-pentapeptide, and glycine. Newly synthesized peptidoglycan is partially cross-linked by a transpeptidation reaction with concomitant release of C-terminal D-alanine. Penicillin not only strongly inhibits release of D-alanine (98% at 1 m...
متن کاملModifications of the acyl-D-alanyl-D-alanine terminus affecting complex-formation with vancomycin.
Vancomycin forms complexes with peptides terminating in d-alanyl-d-alanine that are analogous to the biosynthetic precursors of bacterial mucopeptides. The specificity of complex-formation has been studied by means of many synthetic peptides, prepared by both solid-phase and conventional methods. The following conclusions can be drawn: (a) three amide linkages are required to form a stable comp...
متن کاملThe Significance of D-alanyl-d-alanlne Term1ni in the Biosynthesis of Bacterial Cell Walls and the Action of Penicillin, Vancomyc1n and Ristocet1n
D-Alanyl-D-alanine is a key structure in the biosynthesis of the peptidoglycans. of bacterial cell walls. It is introduced as the last step in the assembly of the precursor nucleotide compound containing muramic acid and remains throughout the biosynthetic process until the terminal D-alanine residue is lost at the final transpeptidation reaction required to effect crosslinking. This transpepti...
متن کاملThe preparation of iodinated vancomycin and its distribution in bacteria treated with the antibiotic.
Vancomycin was radioactively labelled by iodination with (125)I.Iodinated vancomycin was only a little less potent as an antibiotic than vancomycin itself. It was shown, both by chromatography and differential absorption measurements, to combine with acyl-d-alanyl-d-alanine residues. Radioactive vancomycin was used to follow the fate of the antibiotic in bacteria that had been subjected to the ...
متن کاملPolymer length of teichuronic acid released from cell walls of Micrococcus luteus.
Teichuronic acid released from its phosphodiester linkage to peptidoglycan in the cell walls of Micrococcus luteus by mild acid treatment is resolved into a ladderlike series of bands by electrophoresis on polyacrylamide gels in the presence of borate. Each band of the ladder differs from its nearest neighbor by one disaccharide repeat unit, ----4)-2-acetamido-2-deoxy-beta-D-mannopyranuronosyl-...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 5 6 شماره
صفحات -
تاریخ انتشار 1974